A simple method for C-6 modification of guanine nucleosides

A simple method for C-6 modification of guanine nucleosides.

A facile method for the introduction of various substituents at the C-6 position of guanosine and 2'-deoxyguanosine is reported. In a simple, 1-step transformation, tert-butyldimethylsilyl protected guanosine and 2'-deoxyguanosine were converted to the O(6)-(benzotriazol-1-yl) derivatives via reaction with 1H-benzotriazol-1-yloxy-tris(dimethylamino)phosphonium hexafluorophosphate (BOP) and 1,8-...

A Simple, Artistic and Efficient Modification of the Inversion Method

     The simplest and fastest way of producing an acceptable and accurate cast is by adopting the two-pour or inversion method. This process is slightly cumbersome and messy due to difficulty of access. A much more efficient, easy and artistic way of accomplishing it is described by adopting a Rotating Cake Icing Table with a Silicone Baking Mat on top as an autoclavable non-stick barrier.    

Photolabile N-hydroxypyrid-2(1H)-one derivatives of guanine nucleosides: a new method for independent guanine radical generation.

One-electron oxidized guanine is an important reactive intermediate in the formation of oxidatively generated damage in DNA and a variety of methods have been utilized for the abstraction of a single electron from the guanine moiety. In this study, an alternative approach for the site specific, independent generation of the guanine radical, utilizing N-hydroxypyrid-2(1H)-one as a photolabile mo...

A convenient Simple Method for Synthesis of Meta-iodobenzylguanidine (MIBG)

Radioiodinated meta iodobenzylguanidine (MIBG) is one of the important radiopharmaceuticals in Nuclear Medicine. [123/131I]MIBG is used for imaging of Adrenal medulla, studying heart sympathetic nerves, treatment of pheochromacytoma and neuroblastoma. For clinical application, radioiodinated MIBG is prepared through isotopic exchange method, which includes replacement of radioactive iodine in a...

A simple method for the determination of serum cholesterol